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ylbB [2019-02-19 19:52:31]
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ylbB [2019-02-19 19:52:31]

putative oxidoreductase
locus
BSU14950
pI
4.76
mw
16.21 kDa
protein length
148 aa Sequence Blast
gene length
447 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    1,565,849 1,566,295

    The protein

    Paralogous protein(s)

  • [protein|AF3ED8916EA02A560EC8D47E4B1C911E871B9AB0|YhcV]:

    • [SW|Domains]

  • 2 [SW|CBS domain]s (8-68, 74-130)

    • Structure

  • [PDB|4FRY] (protein from ''Burkholderia ambifaria'', 38% identity) [Pubmed|23382856]

    • Expression and Regulation

    Operons

    genes
    [gene|8529D83560E934709B20CCF0B72251A74EC272BB|ylbB]-[gene|2EC813B89149AD39405E66C325FB2684B5987162|ylbC]
    description
    [Pubmed|16497325]

    sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325,15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

    • regulation

  • expressed during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325,15699190]
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    Biological materials

    Mutant

  • MGNA-B242 (ylbB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1241 NBRP B. subtilis, Japan]
  • BKE14950 ([gene|8529D83560E934709B20CCF0B72251A74EC272BB|ylbB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTTTAACCTCCTTTA, downstream forward: _UP4_TAACACATAAATCTTTGACA
  • BKK14950 ([gene|8529D83560E934709B20CCF0B72251A74EC272BB|ylbB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTTTAACCTCCTTTA, downstream forward: _UP4_TAACACATAAATCTTTGACA

    • Expression vectors

  • pGP2934 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jrg Stlke]'s lab

    • References

  • 16497325,15699190,23382856